Abstract
The potential of CD154 (CD40L) as a powerful immunological adjuvant has been shown in various strategies. In this study we examine the immunogenicity and protective efficacy of a CD40-targeting avian influenza hemagglutinin (HA) subunit DNA vaccine in ducks. DNA constructs encoded the ectodomain of the HA protein of LPAI A/mallard/BC/373/2005 (H5N2) with or without fusion to the ectodomain of duck CD154. CD40-targeting significantly accelerated and enhanced humoral responses to the vector-encoded HA protein. In viral challenge experiments with A/chicken/Vietnam/14/2005 (H5N1), DNA immunization conferred partial protection against the genetically distant HPAI. The observed improved kinetics and magnitude of immune induction suggest that CD40-targeting holds promise for influenza A vaccine development.
Copyright © 2010 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animal Structures / pathology
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Animals
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Antibodies, Viral / blood
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CD40 Ligand / genetics
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CD40 Ligand / immunology*
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Cross Protection
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Ducks
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Hemagglutinin Glycoproteins, Influenza Virus / genetics
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Hemagglutinin Glycoproteins, Influenza Virus / immunology*
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Histocytochemistry
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Influenza Vaccines / genetics
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Influenza Vaccines / immunology*
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Influenza in Birds / pathology
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Influenza in Birds / prevention & control*
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Microscopy
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Neutralization Tests
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / immunology
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Vaccines, DNA / genetics
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Vaccines, DNA / immunology*
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Vaccines, Subunit / genetics
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Vaccines, Subunit / immunology
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Vaccines, Synthetic / genetics
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Vaccines, Synthetic / immunology
Substances
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Antibodies, Viral
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Hemagglutinin Glycoproteins, Influenza Virus
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Influenza Vaccines
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Recombinant Fusion Proteins
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Vaccines, DNA
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Vaccines, Subunit
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Vaccines, Synthetic
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hemagglutinin, avian influenza A virus
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CD40 Ligand