Aurora-A overexpression and aneuploidy predict poor outcome in serous ovarian carcinoma

Gynecol Oncol. 2011 Jan;120(1):11-7. doi: 10.1016/j.ygyno.2010.09.003.

Abstract

Objective: Aurora-A is a potential oncogene and therapeutic target in ovarian carcinoma. It is involved in mitotic events and overexpression leads to centrosome amplification and chromosomal instability. The objective of this study was to evaluate the clinical significance of Aurora-A and DNA ploidy in serous ovarian carcinoma.

Methods: Serous ovarian carcinomas were analysed for Aurora-A protein by immunohistochemistry (n=592), Aurora-A copy number by CISH (n=169), Aurora-A mRNA by real-time PCR (n=158) and DNA ploidy by flowcytometry (n=440).

Results: Overexpression of Aurora-A was found in 27% of the tumors, cytoplasmic overexpression in 11% and nuclear in 17%. The cytoplasmic and nuclear overexpression were nearly mutually exclusive. Both cytoplasmic and nuclear overexpression were associated with shorter survival, high grade, high proliferation index and aberrant p53. Interestingly, only cytoplasmic expression was associated with aneuploidy and expression of phosphorylated Aurora-A. DNA ploidy was associated with poor patient outcome as well as aggressive clinicopathological parameters. In multivariate analysis, Aurora-A overexpression appeared as an independent prognostic factor for disease-free survival, together with grade, stage and ploidy.

Conclusions: Aurora-A protein expression is strongly linked with poor patient outcome and aggressive disease characteristics, which makes Aurora-A a promising biomarker and a potential therapeutic target in ovarian carcinoma. Cytoplasmic and nuclear Aurora-A protein may have different functions. DNA aneuploidy is a strong predictor of poor prognosis in serous ovarian carcinoma.

MeSH terms

  • Aneuploidy*
  • Aurora Kinases
  • Cell Nucleus / enzymology
  • Cystadenocarcinoma, Serous / enzymology*
  • Cystadenocarcinoma, Serous / genetics*
  • Cystadenocarcinoma, Serous / pathology
  • Cytoplasm / enzymology
  • DNA Copy Number Variations
  • DNA, Neoplasm / genetics
  • Female
  • Humans
  • Immunohistochemistry
  • In Situ Hybridization
  • Multivariate Analysis
  • Neoplasm Staging
  • Ovarian Neoplasms / enzymology*
  • Ovarian Neoplasms / genetics*
  • Ovarian Neoplasms / pathology
  • Phosphorylation
  • Polymerase Chain Reaction
  • Protein-Serine-Threonine Kinases / biosynthesis*
  • Protein-Serine-Threonine Kinases / genetics
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics

Substances

  • DNA, Neoplasm
  • RNA, Messenger
  • Aurora Kinases
  • Protein-Serine-Threonine Kinases