Identification of estrogen-responsive vitelline envelope protein fragments from rainbow trout (Oncorhynchus mykiss) plasma using mass spectrometry

Mol Reprod Dev. 2010 Nov;77(11):963-70. doi: 10.1002/mrd.21244. Epub 2010 Oct 11.


Plasma peptides previously associated with exposure of juvenile male rainbow trout (Oncorhynchus mykiss) to the hormone 17β-estradiol (E2) were identified using matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS). Specifically, plasma peptides of interest were fractionated and subsequently identified via spectra obtained by MALDI QqTOF MS/MS and LC-MALDI TOFTOF MS/MS analysis, de novo sequencing and database matching. The two peptide masses were identified as significant matches for fragments of the C-terminal propeptides from rainbow trout vitelline envelope protein (VEP)α and VEPγ isoforms. Our findings document the presence of the C-terminal propeptides from rainbow trout VEPα and VEPγ proteins in the bloodstream of juvenile male rainbow trout exposed to E2 via MALDI-TOF-MS detection. We provide three possible explanations for the presence of C-terminal propeptides in the bloodstream, as well as compare previously obtained hepatic transcriptomic results with the plasma proteomic results obtained in the present study.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Biomarkers, Pharmacological / analysis*
  • Biomarkers, Pharmacological / blood
  • Biomarkers, Pharmacological / chemistry
  • Biomarkers, Pharmacological / metabolism
  • Blood Chemical Analysis / methods
  • Egg Proteins / analysis*
  • Egg Proteins / blood
  • Egg Proteins / chemistry
  • Egg Proteins / metabolism
  • Estradiol / pharmacology*
  • Male
  • Mass Spectrometry / methods*
  • Oncorhynchus mykiss / blood*
  • Oncorhynchus mykiss / metabolism
  • Peptide Fragments / analysis
  • Peptide Fragments / blood
  • Peptide Fragments / metabolism
  • Sequence Analysis, Protein / methods
  • Vitelline Membrane / chemistry
  • Vitelline Membrane / drug effects
  • Vitelline Membrane / metabolism


  • Biomarkers, Pharmacological
  • Egg Proteins
  • Peptide Fragments
  • vitelline membrane proteins
  • Estradiol