Chemical composition of five commercial Gynostemma pentaphyllum samples and their radical scavenging, antiproliferative, and anti-inflammatory properties

J Agric Food Chem. 2010 Nov 10;58(21):11243-9. doi: 10.1021/jf1026372. Epub 2010 Oct 12.


Five Gynostemma pentaphyllum (GP) samples were investigated and compared for their chemical compositions and their antioxidant, antiproliferative, and anti-inflammatory effects. Extracts (50% acetone, 75% ethanol, and 100% ethanol) of the five GP samples (GP1-5) differed in their total phenolic, saponin, and flavonoid contents and in their rutin and quercetin concentrations. The highest level of total flavonoids was 63.5 mg of rutin equiv/g in GP4, and the greatest total phenolic content was 44.3 mg of gallic acid equiv/g in GP1 with 50% acetone as the extraction solvent. GP2 had the highest total saponin content of 132.6 mg/g with 100% ethanol as the extraction solvent. These extracts also differed in their scavenging capacity against DPPH and hydroxyl radicals, although they all showed significant radical scavenging capacity. The 100% ethanol extracts also showed dose-dependently strong inhibition on IL-6 and Ptgs2 mRNA expression and weak inhibition on TNF-α mRNA expression. In addition, GP1 had the highest antiproliferative activity at 3.2 mg equiv/mL concentration in HT-29 human colon cancer cells. The results from this study will be used to promote the application of G. pentaphyllum for improving human health.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / chemistry*
  • Anti-Inflammatory Agents / pharmacology
  • Cell Line
  • Cell Proliferation / drug effects
  • Free Radical Scavengers / chemistry*
  • Free Radical Scavengers / pharmacology
  • Growth Inhibitors / chemistry*
  • Growth Inhibitors / pharmacology
  • Gynostemma / chemistry*
  • Humans
  • Interleukin-6 / genetics
  • Interleukin-6 / immunology
  • Macrophages / drug effects
  • Macrophages / immunology
  • Mice
  • Plant Extracts / chemistry*
  • Plant Extracts / pharmacology
  • Tumor Necrosis Factor-alpha / genetics
  • Tumor Necrosis Factor-alpha / immunology


  • Anti-Inflammatory Agents
  • Free Radical Scavengers
  • Growth Inhibitors
  • Interleukin-6
  • Plant Extracts
  • Tumor Necrosis Factor-alpha