TGF-ß1 and FAK regulate periostin expression in PDL fibroblasts

J Dent Res. 2010 Dec;89(12):1439-43. doi: 10.1177/0022034510378684. Epub 2010 Oct 12.


Recently identified as a key component of the murine periodontal ligament (PDL), periostin has been implicated in the regulation of collagen fibrillogenesis and fibroblast differentiation. We investigated whether periostin protein is expressed in the human PDL in situ and the mechanisms regulating periostin expression in PDL fibroblasts in vitro. With immunohistochemistry, periostin protein was identified in the PDL, with expression lower in teeth with reduced occlusal loading. In vitro application of uniaxial cyclic strain to PDL fibroblasts elevated periostin mRNA levels, depending on the age of the patient. Treatment with transforming growth factor-beta1 (TGF-β1) also significantly increased periostin mRNA levels, an effect attenuated by focal adhesion kinase (FAK) inhibition. FAK-null fibroblasts contained no detectable periostin mRNA, even after stimulation with cyclic strain. In conclusion, periostin protein is strongly expressed in the human PDL. In vitro, periostin mRNA levels are modulated by cyclic strain as well as TGF-β1 via FAK-dependent pathways.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Age Factors
  • Bite Force
  • Cell Adhesion Molecules / analysis*
  • Cell Culture Techniques
  • Cells, Cultured
  • Connective Tissue Cells / cytology
  • Fibroblasts / cytology
  • Fibroblasts / drug effects*
  • Focal Adhesion Kinase 1 / antagonists & inhibitors
  • Focal Adhesion Kinase 1 / pharmacology*
  • Humans
  • Immunohistochemistry
  • Middle Aged
  • Periodontal Ligament / cytology
  • Periodontal Ligament / drug effects*
  • Polymerase Chain Reaction / methods
  • Protein-Serine-Threonine Kinases / antagonists & inhibitors
  • RNA, Messenger / analysis
  • Receptor, Transforming Growth Factor-beta Type I
  • Receptors, Transforming Growth Factor beta / antagonists & inhibitors
  • Stress, Mechanical
  • Transforming Growth Factor beta1 / antagonists & inhibitors
  • Transforming Growth Factor beta1 / pharmacology*
  • Young Adult


  • Cell Adhesion Molecules
  • POSTN protein, human
  • RNA, Messenger
  • Receptors, Transforming Growth Factor beta
  • Transforming Growth Factor beta1
  • Focal Adhesion Kinase 1
  • PTK2 protein, human
  • Protein-Serine-Threonine Kinases
  • Receptor, Transforming Growth Factor-beta Type I