Striatal and cortical BOLD, blood flow, blood volume, oxygen consumption, and glucose consumption changes in noxious forepaw electrical stimulation

J Cereb Blood Flow Metab. 2011 Mar;31(3):832-41. doi: 10.1038/jcbfm.2010.173. Epub 2010 Oct 13.


Recent reports showed noxious forepaw stimulation in rats evoked an unexpected sustained decrease in cerebral blood volume (CBV) in the bilateral striatum, whereas increases in spike activity and Fos-immunoreactive cells were observed. This study aimed to further evaluate the hemodynamic and metabolic needs in this model and the sources of negative functional magnetic resonance imaging (fMRI) signals by measuring blood oxygenation-level-dependent (BOLD), cerebral-blood-flow (CBF), CBV, and oxygen-consumption (i.e., cerebral metabolic rate of oxygen (CMRO(2))) changes using an 11.7-T MRI scanner, and glucose-consumption (i.e., cerebral metabolic rate of glucose (CMRglc)) changes using micro-positron emission tomography. In the contralateral somatosensory cortex, BOLD, CBF, CBV, CMRO(2) (n=7, P<0.05), and CMRglc (n=5, P<0.05) increased. In contrast, in the bilateral striatum, BOLD, CBF, and CBV decreased (P<0.05), CMRO(2) decreased slightly, although not significantly from baseline, and CMRglc was not statistically significant from baseline (P>0.05). These multimodal functional imaging findings corroborate the unexpected negative hemodynamic changes in the striatum during noxious forepaw stimulation, and support the hypothesis that striatal hemodynamic response is dominated by neurotransmitter-mediated vasoconstriction, overriding the stimulus-evoked fMRI signal increases commonly accompany elevated neuronal activity. Multimodal functional imaging approach offers a means to probe the unique attributes of the striatum, providing novel insights into the neurovascular coupling in the striatum. These findings may have strong implications in fMRI studies of pain.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Blood Volume*
  • Cerebral Cortex / blood supply*
  • Cerebral Cortex / metabolism
  • Corpus Striatum / blood supply*
  • Corpus Striatum / metabolism
  • Electric Stimulation / methods
  • Fluorodeoxyglucose F18
  • Forelimb
  • Glucose / metabolism*
  • Magnetic Resonance Imaging
  • Male
  • Oxygen / blood*
  • Oxygen Consumption*
  • Pain / physiopathology*
  • Positron-Emission Tomography
  • Radiopharmaceuticals
  • Rats
  • Rats, Sprague-Dawley
  • Regional Blood Flow


  • Radiopharmaceuticals
  • Fluorodeoxyglucose F18
  • Glucose
  • Oxygen