Loss of WNT-TCF addiction and enhancement of HH-GLI1 signalling define the metastatic transition of human colon carcinomas

EMBO Mol Med. 2010 Nov;2(11):440-57. doi: 10.1002/emmm.201000098.

Abstract

Previous studies demonstrate the initiation of colon cancers through deregulation of WNT-TCF signalling. An accepted but untested extension of this finding is that incurable metastatic colon carcinomas (CCs) universally remain WNT-TCF-dependent, prompting the search for WNT-TCF inhibitors. CCs and their stem cells also require Hedgehog (HH)-GLI1 activity, but how these pathways interact is unclear. Here we define coincident high-to-low WNT-TCF and low-to-high HH-GLI transitions in patient CCs, most strikingly in their CD133(+) stem cells, that mark the development of metastases. We find that enhanced HH-GLI mimics this transition, driving also an embryonic stem (ES)-like stemness signature and that GLI1 can be regulated by multiple CC oncogenes. The data support a model in which the metastatic transition involves the acquisition or enhancement of a more primitive ES-like phenotype, and the downregulation of the early WNT-TCF programme, driven by oncogene-regulated high GLI1 activity. Consistently, TCF blockade does not generally inhibit tumour growth; instead, it, like enhanced HH-GLI, promotes metastatic growth in vivo. Treatments for metastatic disease should therefore block HH-GLI1 but not WNT-TCF activities.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • AC133 Antigen
  • Antigens, CD / analysis
  • Carcinoma / pathology
  • Carcinoma / physiopathology*
  • Colonic Neoplasms / pathology
  • Colonic Neoplasms / physiopathology*
  • Gene Expression Profiling
  • Glycoproteins / analysis
  • Hedgehog Proteins / metabolism*
  • Humans
  • Neoplasm Metastasis / pathology
  • Neoplasm Metastasis / physiopathology*
  • Peptides / analysis
  • Stem Cells
  • TCF Transcription Factors / metabolism*
  • Transcription Factors / metabolism*
  • Wnt Proteins / metabolism*
  • Zinc Finger Protein GLI1

Substances

  • AC133 Antigen
  • Antigens, CD
  • GLI1 protein, human
  • Glycoproteins
  • Hedgehog Proteins
  • PROM1 protein, human
  • Peptides
  • TCF Transcription Factors
  • Transcription Factors
  • Wnt Proteins
  • Zinc Finger Protein GLI1