Therapeutic cold: An effective kind to modulate the oxidative damage resulting of a skeletal muscle contusion

Free Radic Res. 2011 Feb;45(2):125-38. doi: 10.3109/10715762.2010.517252. Epub 2010 Oct 13.


Muscular contusions affect the function of the skeletal muscle system. This study investigated the oxidative damage as well as the main morphological changes related to a skeletal muscle contusion in the gastrocnemius muscle of rats and also the capacity of therapeutic cold to modulate these parameters. The therapeutic cold modulated the increase of oxidative stress markers and also modulated the reduction in the antioxidants levels in the injured muscle. In enzyme assays, therapeutic cold was also effective in normalizing the muscle Na(+)/K(+) and Ca(2+) ATPases, lactate dehydrogenase and myeloperoxidase activities. Similarly, the lesioned non-treated animals presented evident impairments in the mitochondrial functions and in the muscle morphology which were diminished by the cold treatment. The therapeutic cold was able to modulate the oxidative damage possibly by its capacity to limit the inflammatory response intensity, to attenuate the impairment of the mitochondrial function and also to preserve the skeletal muscle morphology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomarkers / analysis*
  • Biomarkers / metabolism
  • Calcium-Transporting ATPases / metabolism
  • Cold Temperature
  • Contusions / metabolism*
  • Cryotherapy*
  • L-Lactate Dehydrogenase / metabolism
  • Male
  • Membrane Potential, Mitochondrial
  • Mitochondria, Muscle / metabolism*
  • Muscle, Skeletal / injuries
  • Muscle, Skeletal / metabolism*
  • Muscle, Skeletal / physiopathology
  • Oxidation-Reduction
  • Oxidative Stress
  • Peroxidase / metabolism
  • Rats
  • Rats, Wistar
  • Sodium-Potassium-Exchanging ATPase / metabolism
  • Staining and Labeling


  • Biomarkers
  • L-Lactate Dehydrogenase
  • Peroxidase
  • Calcium-Transporting ATPases
  • Sodium-Potassium-Exchanging ATPase