Exome Sequencing, ANGPTL3 Mutations, and Familial Combined Hypolipidemia

N Engl J Med. 2010 Dec 2;363(23):2220-7. doi: 10.1056/NEJMoa1002926. Epub 2010 Oct 13.

Abstract

We sequenced all protein-coding regions of the genome (the "exome") in two family members with combined hypolipidemia, marked by extremely low plasma levels of low-density lipoprotein (LDL) cholesterol, high-density lipoprotein (HDL) cholesterol, and triglycerides. These two participants were compound heterozygotes for two distinct nonsense mutations in ANGPTL3 (encoding the angiopoietin-like 3 protein). ANGPTL3 has been reported to inhibit lipoprotein lipase and endothelial lipase, thereby increasing plasma triglyceride and HDL cholesterol levels in rodents. Our finding of ANGPTL3 mutations highlights a role for the gene in LDL cholesterol metabolism in humans and shows the usefulness of exome sequencing for identification of novel genetic causes of inherited disorders. (Funded by the National Human Genome Research Institute and others.).

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiopoietin-like Proteins
  • Angiopoietins / genetics*
  • Cholesterol, HDL / blood
  • Cholesterol, HDL / genetics
  • Cholesterol, LDL / blood
  • Cholesterol, LDL / genetics
  • Codon, Nonsense*
  • DNA Mutational Analysis
  • Female
  • Genetic Linkage
  • Humans
  • Hypobetalipoproteinemias / genetics*
  • Male
  • Pedigree

Substances

  • ANGPTL3 protein, human
  • Angiopoietin-like Proteins
  • Angiopoietins
  • Cholesterol, HDL
  • Cholesterol, LDL
  • Codon, Nonsense