Super-highly hydroxylated fullerene derivative protects human keratinocytes from UV-induced cell injuries together with the decreases in intracellular ROS generation and DNA damages

J Photochem Photobiol B. 2011 Jan 10;102(1):69-76. doi: 10.1016/j.jphotobiol.2010.09.006. Epub 2010 Oct 1.

Abstract

Polyhydroxylated fullerenes (fullerenols: C(60)(OH)(n)) are known as the major water-soluble fullerene derivatives which possess particular significance as free radical scavengers or antioxidants in biological systems. Recently, the novel polyhydroxylated fullerene (C(60) (OH)(44)·8H(2)O: SHH-F) was successfully synthesized. In the present study, we investigated the radical-scavenging effects and cytoprotective effects of three types of fullerenols (C(60)(OH)(6-12): LH-F, C(60) (OH)(32-34)·7H(2)O: HH-F, and C(60) (OH)(44)·8H(2)O: SHH-F) on UV-irradiation-induced cell injuries. HH-F and SHH-F exerted hydroxyl-radical scavenging activities as shown by DMPO-spin trap/ESR method, more markedly than LH-F. UVA or UVB irradiation-induced injuries in human skin keratinocytes HaCaT were significantly suppressed by HH-F and SHH-F, but scarcely by LF-H. The cytoprotective effects of SHH-F had a tendency to be superior to that of HH-F. And the cytoprotective effects of SHH-F against UVB-induced injuries were more effective than those of UVA. Irradiation with UVB to HaCaT cells was shown to cause rapid increases in cell-injury-associated symptoms such as intracellular oxidative stress levels, the formation of cyclobutane pyrimidine dimers and chromatin condensation, all of which were repressed by SHH-F. Thus, UVB-induced diverse harmful effects could be prevented by SHH-F, which was suggested to exert the cytoprotective effects through intracellular reactive oxygen species-scavenging in the keratinocytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatin / drug effects
  • Chromatin / metabolism
  • Cytoprotection / drug effects
  • DNA Damage*
  • Free Radical Scavengers / chemistry
  • Free Radical Scavengers / pharmacology
  • Fullerenes / chemistry*
  • Fullerenes / pharmacology*
  • Humans
  • Hydroxyl Radical / metabolism
  • Hydroxylation
  • Intracellular Space / drug effects
  • Intracellular Space / genetics
  • Intracellular Space / metabolism*
  • Intracellular Space / radiation effects
  • Keratinocytes / cytology*
  • Keratinocytes / drug effects
  • Keratinocytes / metabolism
  • Keratinocytes / radiation effects
  • Pyrimidine Dimers / metabolism
  • Reactive Oxygen Species / metabolism*
  • Ultraviolet Rays / adverse effects*

Substances

  • Chromatin
  • Free Radical Scavengers
  • Fullerenes
  • Pyrimidine Dimers
  • Reactive Oxygen Species
  • fullerenol
  • Hydroxyl Radical
  • fullerene C60