Extension of the Lifespan of Caenorhabditis Elegans by the Use of Electrolyzed Reduced Water

Biosci Biotechnol Biochem. 2010;74(10):2011-5. doi: 10.1271/bbb.100250. Epub 2010 Oct 7.

Abstract

Electrolyzed reduced water (ERW) has attracted much attention because of its therapeutic effects. In the present study, a new culture medium, which we designated Water medium, was developed to elucidate the effects of ERW on the lifespan of Caenorhabditis elegans. Wild-type C. elegans had a significantly shorter lifespan in Water medium than in conventional S medium. However, worms cultured in ERW-Water medium exhibited a significantly extended lifespan (from 11% to 41%) compared with worms cultured in ultrapure water-Water medium. There was no difference between the lifespans of worms cultured in ERW-S medium and ultrapure water-S medium. Nematodes cultured in ultrapure water-Water medium showed significantly higher levels of reactive oxygen species than those cultured in ultrapure water-S medium. Moreover, ERW-Water medium significantly reduced the ROS accumulation induced in the worms by paraquat, suggesting that ERW-Water medium extends the longevity of nematodes at least partly by scavenging ROS.

MeSH terms

  • Animals
  • Caenorhabditis elegans / drug effects*
  • Caenorhabditis elegans / metabolism
  • Caenorhabditis elegans / physiology*
  • Culture Media / chemistry
  • Culture Media / pharmacology
  • Electrolysis*
  • Longevity / drug effects*
  • Reactive Oxygen Species / metabolism
  • Water / chemistry*
  • Water / pharmacology*

Substances

  • Culture Media
  • Reactive Oxygen Species
  • Water