Cell-specific regulation of Fas exon 6 splicing mediated by Hu antigen R

Biochem Biophys Res Commun. 2010 Nov 12;402(2):324-8. doi: 10.1016/j.bbrc.2010.10.025. Epub 2010 Oct 15.

Abstract

The differential expression levels of T-cell intracellular antigens (TIA) and Hu antigen R (HuR) are concomitant with a splicing switch in apoptosis receptor Fas in HCT-116 cells. Thus, overexpression and knockdown of HuR led to Fas exon 6 skipping and inclusion, respectively. These results suggest that the TIA and HuR cellular ratio influences cell-type specific Fas exon 6 splicing pattern.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing*
  • Antigens, Surface / genetics
  • Antigens, Surface / metabolism*
  • Cell Line
  • Cell Line, Tumor
  • ELAV Proteins
  • ELAV-Like Protein 1
  • Exons / genetics*
  • Gene Knockdown Techniques
  • HeLa Cells
  • Humans
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism*
  • fas Receptor / genetics*

Substances

  • Antigens, Surface
  • ELAV Proteins
  • ELAV-Like Protein 1
  • ELAVL1 protein, human
  • FAS protein, human
  • RNA-Binding Proteins
  • fas Receptor