Dual RMCE for efficient re-engineering of mouse mutant alleles

Nat Methods. 2010 Nov;7(11):893-5. doi: 10.1038/nmeth.1521. Epub 2010 Oct 17.


We have developed dual recombinase-mediated cassette exchange (dRMCE) to efficiently re-engineer the thousands of available conditional alleles in mouse embryonic stem cells. dRMCE takes advantage of the wild-type loxP and FRT sites present in these conditional alleles and in many gene-trap lines. dRMCE is a scalable, flexible tool to introduce tags, reporters and mutant coding regions into an endogenous locus of interest in an easy and highly efficient manner.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Animals
  • Basic Helix-Loop-Helix Transcription Factors / genetics
  • Carrier Proteins / physiology
  • Embryonic Stem Cells / metabolism*
  • Genetic Engineering / methods*
  • Intracellular Signaling Peptides and Proteins
  • Mice
  • Nerve Tissue Proteins / physiology
  • Nuclear Proteins / physiology
  • Promoter Regions, Genetic
  • Recombinases / physiology*
  • Recombination, Genetic
  • Smad4 Protein / genetics


  • Basic Helix-Loop-Helix Transcription Factors
  • Carrier Proteins
  • Hand2 protein, mouse
  • Intracellular Signaling Peptides and Proteins
  • Nerve Tissue Proteins
  • Nuclear Proteins
  • Recombinases
  • Smad4 Protein
  • Smad4 protein, mouse
  • Zfp503 protein, mouse