12/15-lipoxygenase is an interleukin-13 and interferon-γ counterregulated-mediator of allergic airway inflammation

Mediators Inflamm. 2010:2010:727305. doi: 10.1155/2010/727305. Epub 2010 Sep 29.

Abstract

Interleukin-13 and interferon-γ are important effectors of T-helper cells. Interleukin-13 increases expression of the arachidonic acid-metabolizing enzyme, 15-lipoxygenase-1, in a variety of cell types. 15-lipoxygenase-1 is dramatically elevated in the airways of subjects with asthma. Studies in animals indicate that 15-lipoxygenase-1 contributes to the development of allergic airway inflammation but is protective in some other forms of inflammation. We tested the hypothesis that the ability of interleukin-13 and interferon-γ to counterregulate allergic airway inflammation was potentially mediated by counterregulation of 12/15-lipoxygenase, the mouse ortholog of 15-lipoxygenase-1. The airways of mice were treated with interleukin-13 or interferon-γ one day prior to each of the four allergen exposures. Interleukin-13 augmented and interferon-γ inhibited allergic airway inflammation independently of systemic IgE and mucosal IgA responses but in association with counterregulation of 12/15-lipoxygenase. Interleukin-13 and interferon-γ counterregulate 12/15-lipoxygenase potentially contributing to the effects of these cytokines on allergic airway inflammation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allergens / metabolism
  • Animals
  • Arachidonate 12-Lipoxygenase / metabolism*
  • Arachidonate 15-Lipoxygenase / metabolism*
  • Asthma / metabolism*
  • Cytokines / metabolism
  • Gene Expression Regulation*
  • Humans
  • Hypersensitivity
  • Immunoglobulin A / metabolism
  • Immunoglobulin E / metabolism
  • Inflammation
  • Interferon-gamma / metabolism*
  • Interleukin-13 / metabolism*
  • Mice
  • Mice, Inbred C57BL

Substances

  • 12-15-lipoxygenase
  • Allergens
  • Cytokines
  • Immunoglobulin A
  • Interleukin-13
  • Immunoglobulin E
  • Interferon-gamma
  • Arachidonate 12-Lipoxygenase
  • Arachidonate 15-Lipoxygenase