Mandibular segmental defect regenerated with macroporous biphasic calcium phosphate, collagen membrane, and bone marrow graft in dogs

Arch Otolaryngol Head Neck Surg. 2010 Oct;136(10):971-8. doi: 10.1001/archoto.2010.173.


Objective: To reconstruct segmental mandibulectomy using calcium phosphate ceramics and collagen membrane with a delayed bone marrow grafting in experimental animals.

Design: Defects of segmental mandibulectomy were filled with calcium phosphate granules and wrapped with a collagen membrane in 4 dogs and left empty as a control in 2 dogs. Two months later, a bone marrow graft was injected into the center of the implants. Animals were humanely killed after a 16-week delay.

Subjects: Six adult beagles were included in this study.

Intervention: Segmental mandibulectomy.

Main outcome measure: Bone ingrowth and material resorption in the reconstructed segment.

Results: Successful osseous colonization bridged the whole length of the defects. The good new bone formation at the center and the periosteum-like formation at the periphery suggest the osteoinductive role of the bone marrow graft and the healing scaffold role of the membrane.

Conclusions: This model succeeded in regenerating a large segmental defect in the mandible. An investigation with a postimplantation radiation delivery schedule is required with the use of this model, which should be considered as a preclinical study for a bone tissue engineering approach in patients with cancer-related bone defects.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Absorbable Implants
  • Animals
  • Bone Marrow Transplantation*
  • Calcium Phosphates / administration & dosage*
  • Collagen*
  • Dogs
  • Female
  • Guided Tissue Regeneration
  • Mandible / physiology
  • Mandible / surgery*
  • Membranes, Artificial*
  • Microscopy, Electron, Scanning
  • Models, Animal
  • Osteogenesis


  • Calcium Phosphates
  • Membranes, Artificial
  • Collagen
  • calcium phosphate