Proteasome inhibitors induce nucleolar aggregation of proteasome target proteins and polyadenylated RNA by altering ubiquitin availability

Oncogene. 2011 Feb 17;30(7):790-805. doi: 10.1038/onc.2010.469. Epub 2010 Oct 18.

Abstract

The ubiquitin-proteasome pathway is essential for most cellular processes, including protein quality control, cell cycle, transcription, signaling, protein transport, DNA repair and stress responses. Hampered proteasome activity leads to the accumulation of polyubiquitylated proteins, endoplastic reticulum (ER) stress and even cell death. The ability of chemical proteasome inhibitors (PIs) to induce apoptosis is utilized in cancer therapy. During PI treatment, misfolded proteins accrue to cytoplasmic aggresomes. The formation of aggresome-like structures in the nucleus has remained obscure. We identify here a nucleolus-associated RNA-protein aggregate (NoA) formed by the inhibition of proteasome activity in mammalian cells. The aggregate forms within the nucleolus and is dependent on nucleolar integrity, yet is a separate structure, lacking nucleolar marker proteins, ribosomal RNA (rRNA) and rRNA synthesis activity. The NoAs contain polyadenylated RNA, conjugated ubiquitin and numerous nucleoplasmic proteasome target proteins. Several of these are key factors in oncogenesis, including transcription factors p53 and retinoblastoma protein (Rb), several cell cycle-regulating cyclins and cyclin-dependent kinases (CDKs), and stress response kinases ataxia-telangiectasia mutated (ATM) and Chk1. The aggregate formation depends on ubiquitin availability, as shown by modulating the levels of ubiquitin and deubiquitinases. Furthermore, inhibition of chromosome region maintenance 1 protein homolog (CRM1) export pathway aggravates the formation of NoAs. Taken together, we identify here a novel nuclear stress body, which forms upon proteasome inactivity within the nucleolus and is detectable in mammalian cell lines and in human tissue. These findings show that the nucleolus controls protein and RNA surveillance and export by the ubiquitin pathway in a previously unidentified manner, and provide mechanistic insight into the cellular effects of PIs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylcysteine / analogs & derivatives
  • Acetylcysteine / pharmacology
  • Ataxia Telangiectasia Mutated Proteins
  • Cell Cycle Proteins / metabolism
  • Cell Line
  • Cell Nucleolus / drug effects
  • Cell Nucleolus / metabolism
  • Checkpoint Kinase 1
  • Cyclin-Dependent Kinases / metabolism
  • Cyclins / metabolism
  • Cysteine Proteinase Inhibitors / pharmacology*
  • DNA-Binding Proteins / metabolism
  • Humans
  • Leucine / analogs & derivatives
  • Leucine / metabolism
  • Leupeptins / pharmacology
  • Nuclear Proteins / metabolism*
  • Proteasome Endopeptidase Complex / metabolism*
  • Proteasome Inhibitors*
  • Protein Kinases / metabolism
  • Protein-Serine-Threonine Kinases / metabolism
  • RNA, Messenger / metabolism
  • Transcription Factors / metabolism
  • Tumor Suppressor Proteins / metabolism
  • Ubiquitin / metabolism*

Substances

  • Cell Cycle Proteins
  • Cyclins
  • Cysteine Proteinase Inhibitors
  • DNA-Binding Proteins
  • Leupeptins
  • Nuclear Proteins
  • Proteasome Inhibitors
  • RNA, Messenger
  • Transcription Factors
  • Tumor Suppressor Proteins
  • Ubiquitin
  • acetylleucyl-leucyl-norleucinal
  • lactacystin
  • Protein Kinases
  • ATM protein, human
  • Ataxia Telangiectasia Mutated Proteins
  • CHEK1 protein, human
  • Checkpoint Kinase 1
  • Protein-Serine-Threonine Kinases
  • Cyclin-Dependent Kinases
  • Proteasome Endopeptidase Complex
  • Leucine
  • leupeptin
  • E 64
  • benzyloxycarbonylleucyl-leucyl-leucine aldehyde
  • Acetylcysteine