High glucose promotes cell proliferation and enhances GDNF and RET expression in pancreatic cancer cells

Mol Cell Biochem. 2011 Jan;347(1-2):95-101. doi: 10.1007/s11010-010-0617-0. Epub 2010 Oct 20.


Hyperglycemia promotes pancreatic cancer progression, while the underlying mechanism is uncertain. We investigated the cell proliferation, glial cell line-derived neurotrophic factor (GDNF) and its tyrosine kinase receptor RET expression in BxPC-3 and MIA PaCa-2 cells when exposed to different concentrations of glucose. Proliferation of both cells was effected by glucose in a concentration-dependent manner. Definite expression of GDNF and RET was detected in both cells. Glucose concentrations could alter the expression of GDNF and RET in a concentration-dependent manner, correspondingly with the alterations of cell proliferation. Up-regulation of GDNF and RET ligand-receptor interaction might participate in the glucose-induced cancer progression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Densitometry
  • Gene Expression Regulation, Neoplastic / drug effects
  • Glial Cell Line-Derived Neurotrophic Factor / genetics
  • Glial Cell Line-Derived Neurotrophic Factor / metabolism*
  • Glucose / pharmacology*
  • Humans
  • Pancreatic Neoplasms / genetics
  • Pancreatic Neoplasms / pathology*
  • Proto-Oncogene Proteins c-ret / genetics
  • Proto-Oncogene Proteins c-ret / metabolism*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism


  • GDNF protein, human
  • Glial Cell Line-Derived Neurotrophic Factor
  • RNA, Messenger
  • Proto-Oncogene Proteins c-ret
  • RET protein, human
  • Glucose