Local antigonadotrophic action of melatonin in testes has never been correlated with local and general immune status of any rodent. Intra-testicular injection of melatonin (2.5 μg/50 μl) for 10 days (MI-10D) and 20 days (MI-20D) was given to young adult male of Funambulus pennanti and testicular androgen receptor (AR), androgen binding protein (ABP) expression, 3β-hydroxysteroid dehydrogenase (3β-HSD) activity, and Mel1aR expression in thymus was checked along with general immune parameters. Further, immunohistochemical localization of Mel1aR in testes was done. Decreased AR, ABP expression, testes weight, 3β-HSD activity, testosterone level, and spermatogenesis but increased Mel1aR expression in thymus, immunoreactivity in testes, and testicular macrophages following injection was noted. Lymphatic tissue weight, leukocyte, lymphocyte count, lymphocyte proliferation in spleen, thymus, plasma melatonin, and IL-2 level increased in a duration-dependent manner following intra-testicular injection. Intra-testicular injection of melatonin decreased steroidogenesis by enhancing the primary effect of melatonin on Leydig cell endocrine function. Along with reduced circulatory testosterone production, an increase in testicular as well as general immunity was observed in a duration-dependent manner. Therefore, a local participation of melatonin in testes of F. pennanti to control testicular androgen production is suggested.