Micromolding for the fabrication of biological microarrays

Methods Mol Biol. 2011;671:249-60. doi: 10.1007/978-1-59745-551-0_15.

Abstract

The PRINT(®) (pattern replication in non-wetting templates) process has been developed as a simple, gentle way to pattern films or generate discrete particles in arrays out of either pure biological materials or biomolecules encapsulated within polymeric materials. Patterned films and particle arrays can be fabricated in a wide array of sizes and shapes using Fluorocur(®) (a UV-curable perfluoropolyether polymer) from the nanometer to micron scale.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Avidin / chemistry
  • Avidin / metabolism
  • Ethers / chemistry*
  • Fluorocarbons / chemistry*
  • Humans
  • Microarray Analysis / instrumentation*
  • Microtechnology / methods*
  • Nanoparticles / chemistry
  • Nanoparticles / ultrastructure
  • Nanotechnology / methods
  • Oligonucleotides / chemistry*
  • Polyethylene Glycols / chemistry
  • Polymers / chemistry*
  • Proteins / chemistry*
  • Proteins / metabolism
  • Surface Properties

Substances

  • Ethers
  • Fluorocarbons
  • Oligonucleotides
  • Polymers
  • Proteins
  • perfluoropolyether
  • Avidin
  • Polyethylene Glycols