[Long-lived radicals of amino acids induced by X-ray-radiation are the source of hydrogen peroxide in aqueous medium]

Biofizika. 2010 Jul-Aug;55(4):588-93.
[Article in Russian]

Abstract

The formation of long-lived radicals in the solutions of casein and its hydrolysate with an equimolar mixture of amino acids was compared by measuring the X-ray-induced chemiluminescence. It was shown that free amino acids constituting the protein produce long-lived radicals. It was demonstrated that some amino acids (Leu, Ile, Val, Ser, Trp, Met, Pro, Arg, Gly, Phe) emit light of visible spectrum over a long period of time after the irradiation, which indicates the generation of long-lived radicals of these amino acids. The half-life times of these radicals are several hours. Dissolving irradiated dry amino acids capable of luminescing over a long time gives rise to the formation of hydrogen peroxide in aqueous medium.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids / chemistry
  • Amino Acids / radiation effects*
  • Caseins / chemistry
  • Caseins / radiation effects
  • Free Radicals / chemistry
  • Hydrogen Peroxide / chemistry*
  • Hydrolysis
  • Luminescent Measurements
  • Protein Conformation
  • Water / chemistry*

Substances

  • Amino Acids
  • Caseins
  • Free Radicals
  • Water
  • Hydrogen Peroxide