Correlation of microperimetry with fundus autofluorescence and spectral-domain optical coherence tomography in repaired macular holes

Am J Ophthalmol. 2011 Jan;151(1):128-136.e3. doi: 10.1016/j.ajo.2010.06.040. Epub 2010 Oct 20.


Purpose: To investigate the correlation of microperimetry with fundus autofluorescence (FAF), spectral-domain optical coherence tomography (SD-OCT), and visual acuity (VA) in order to better characterize visual outcomes after successful macular hole (MH) surgery.

Design: Cross-sectional case series.

Methods: Postoperative VA, microperimetry, FAF, and SD-OCT images from 23 eyes of 23 patients who underwent successful MH surgery were obtained. FAF images were examined using the Heidelberg retina angiograph 2, and foveal structure and macular sensitivity were evaluated with SD-OCT and microperimetry. The mean retinal sensitivities within the central 9 degrees (microperimetry, mean), the retinal sensitivity of the foveal center (microperimetry, center), and the difference between the values obtained for the foveal center or mean of study and fellow eyes (microperimetry, centerdiff and microperimetry, meandiff, respectively) were measured with microperimetry.

Results: Microperimetry (mean) was well correlated with microperimetry (center) in both study and fellow eyes. Poor postoperative VA was correlated with large microperimetry (meandiff). Following successful MH surgery, FAF of all eyes decreased markedly. There was a positive correlation between microperimetry (centerdiff) and degree of FAF of study eyes. However, a decrease in FAF after MH surgery was not correlated well with either degree of defect in the junction between photoreceptor inner and outer segment (IS/OS) or central retinal thickness on SD-OCT.

Conclusions: The amount of remaining FAF is related to macular sensitivity as measured by microperimetry after successful MH surgery. Function of photoreceptors and retinal pigment epithelium as well as integrity can be estimated by measuring the decrease in FAF after successful MH surgery. Moreover, functional correlation with microperimetry provides both morphologic and functional information on repaired MHs.

MeSH terms

  • Aged
  • Basement Membrane / surgery
  • Cross-Sectional Studies
  • Female
  • Fluorescein Angiography*
  • Fluorocarbons / administration & dosage
  • Fundus Oculi
  • Humans
  • Male
  • Middle Aged
  • Retina / physiopathology
  • Retinal Perforations / physiopathology*
  • Retinal Perforations / surgery*
  • Tomography, Optical Coherence*
  • Visual Acuity / physiology*
  • Visual Field Tests*
  • Vitrectomy


  • Fluorocarbons
  • perflutren