A novel hydrogen sulfide donor causes stomatal opening and reduces nitric oxide accumulation

Plant Physiol Biochem. 2010 Dec;48(12):931-5. doi: 10.1016/j.plaphy.2010.09.016. Epub 2010 Oct 1.


Effects of hydrogen sulfide (H(2)S) on plant physiology have been previously studied, but such studies have relied on the use of NaSH as a method for supplying H(2)S to tissues. Now new compounds which give a less severe H(2)S shock and a more prolonged exposure to H(2)S have been developed. Here the effects of one such compound, GYY4137, has been investigated to determine its effects on stomatal closure in Arabidopsis thaliana. It was found that both NaSH and GYY4137 caused stomatal opening in the light and prevented stomatal closure in the dark. Nitric oxide (NO) has been well established as a mediator of stomatal movements and here it was found that both NaSH and GYY4137 reduced the accumulation of NO in guard cells, perhaps suggesting a mode of action for H(2)S in this system. GYY4137, and future related compounds, will be important tools to unravel the effects of plant exposure to H(2)S and to determine how H(2)S may fit into plant cell signalling pathways.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / drug effects*
  • Arabidopsis / physiology
  • Darkness
  • Hydrogen Sulfide / pharmacology*
  • Light
  • Morpholines / pharmacology*
  • Nitric Oxide / metabolism*
  • Organothiophosphorus Compounds / pharmacology*
  • Plant Stomata / drug effects*
  • Plant Stomata / physiology
  • Sulfhydryl Compounds / pharmacology*


  • GYY 4137
  • Morpholines
  • Organothiophosphorus Compounds
  • Sulfhydryl Compounds
  • Nitric Oxide
  • Hydrogen Sulfide