Enteral diets enriched with medium-chain triglycerides and N-3 fatty acids prevent chemically induced experimental colitis in rats

Transl Res. 2010 Nov;156(5):282-91. doi: 10.1016/j.trsl.2010.07.012. Epub 2010 Aug 24.


The specific purpose of this study was to evaluate the significant effects of medium-chain triglycerides (MCTs) and N-3 fatty acids on chemically induced experimental colitis induced by 2,4,6-trinitrobenzene sulphonic acid (TNBS) in rats. Male Wistar rats were fed liquid diets enriched with N-6 fatty acid (control diets), N-3 fatty acid (MCT- diets), and N-3 fatty acid and MCT (MCT+ diets) for 2 weeks and then were given an intracolonic injection of TNBS. Serum and tissue samples were collected 5 days after ethanol or TNBS enema. The severity of colitis was evaluated pathologically, and tissue myeloperoxidase activity was measured in colonic tissues. Furthermore, protein levels for inflammatory cytokines and a chemokine were assessed by an enzyme-linked immunosorbent assay in colonic tissues. Induction of proinflammatory cytokines tumor necrosis factor-α and interleukin-1β in the colon by TNBS enema was markedly attenuated by the MCT+ diet among the 3 diets studied. Furthermore, the induction of chemokines macrophage inflammatory protein-2 and monocyte chemotactic protein-1 also was blunted significantly in animals fed the MCT+ diets. As a result, MPO activities in the colonic tissue also were blunted significantly in animals fed the MCT+ diets compared with those fed the control diets or the MCT- diets. Furthermore, the MCT+ diet improved chemically induced colitis significantly among the 3 diets studied. Diets enriched with both MCTs and N-3 fatty acids may be effective for the therapy of inflammatory bowel disease as antiinflammatory immunomodulating nutrients.

MeSH terms

  • Animal Feed
  • Animals
  • Body Weight / drug effects
  • Chemokines / metabolism
  • Colitis / chemically induced
  • Colitis / pathology
  • Colitis / prevention & control*
  • Colon / drug effects
  • Colon / enzymology
  • Colon / pathology
  • Disease Models, Animal
  • Endotoxemia / blood
  • Endotoxemia / drug therapy
  • Endotoxemia / etiology
  • Endotoxins / blood
  • Enema
  • Enteral Nutrition / methods*
  • Fatty Acids, Omega-3 / administration & dosage*
  • Male
  • Peroxidase / metabolism
  • Rats
  • Rats, Wistar
  • Triglycerides / administration & dosage*
  • Trinitrobenzenesulfonic Acid / toxicity


  • Chemokines
  • Endotoxins
  • Fatty Acids, Omega-3
  • Triglycerides
  • Trinitrobenzenesulfonic Acid
  • Peroxidase