Reverse phase protein microarrays advance to use in clinical trials

Mol Oncol. 2010 Dec;4(6):461-81. doi: 10.1016/j.molonc.2010.09.003. Epub 2010 Oct 16.

Abstract

Individualizing cancer therapy for molecular targeted inhibitors requires a new class of molecular profiling technology that can map the functional state of the cancer cell signal pathways containing the drug targets. Reverse phase protein microarrays (RPMA) are a technology platform designed for quantitative, multiplexed analysis of specific phosphorylated, cleaved, or total (phosphorylated and non-phosphorylated) forms of cellular proteins from a limited amount of sample. This class of microarray can be used to interrogate tissue samples, cells, serum, or body fluids. RPMA were previously a research tool; now this technology has graduated to use in research clinical trials with clinical grade sensitivity and precision. In this review we describe the application of RPMA for multiplexed signal pathway analysis in therapeutic monitoring, biomarker discovery, and evaluation of pharmaceutical targets, and conclude with a summary of the technical aspects of RPMA construction and analysis.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Antineoplastic Agents / therapeutic use
  • Biomarkers, Tumor / analysis*
  • Clinical Trials as Topic*
  • Drug Discovery
  • Humans
  • Neoplasm Proteins / analysis*
  • Neoplasms / drug therapy
  • Neoplasms / metabolism*
  • Precision Medicine
  • Protein Array Analysis / instrumentation
  • Protein Array Analysis / methods*
  • Protein Array Analysis / standards
  • Proteomics / instrumentation
  • Proteomics / methods
  • Signal Transduction
  • Translational Medical Research

Substances

  • Antineoplastic Agents
  • Biomarkers, Tumor
  • Neoplasm Proteins