Gsdma3 is required for hair follicle differentiation in mice

Biochem Biophys Res Commun. 2010 Dec 3;403(1):18-23. doi: 10.1016/j.bbrc.2010.10.094. Epub 2010 Oct 25.

Abstract

Hair follicle differentiation is regulated by multiple signaling pathways. However, the known cellular and molecular mechanisms are limited. Gsdma3 is a novel murine gene and considered to be a mutation hotspot. Six mutants have been reported in Gsdma3 and all these mutants exhibit hair loss and hyperkeratosis phenotypes. In order to verify how the lack of Gsdma3 affects the hair defects, we use alopecia and excoriation mice, a new mouse mutation in this gene, as our research model. This mutation exhibits progressive hair loss, from head to the whole back, and followed by hair regrowth. We test that Gsdma3 is expressed in matrix, inner root sheath, and hair shaft. Ultrastructural and histological analyses show abnormal hair structures and reduced hair keratins in AE mice. The loss of interlocking structures and abnormal constitutive protein indicate defects in anchoring hair shaft in the hair follicle and resisting external forces. Molecular analysis of Gsdma3 deficiency and overexpression shows an Msx2/Foxn1/acidic hair keratin genetic pathway is involved. Thus, Gsdma3 is necessary for normal hair follicle differentiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alopecia / genetics
  • Animals
  • Cell Differentiation*
  • Forkhead Transcription Factors / metabolism
  • Hair Follicle / abnormalities
  • Hair Follicle / cytology*
  • Homeodomain Proteins / metabolism*
  • Mice
  • Mice, Mutant Strains
  • Proteins / genetics
  • Proteins / metabolism*
  • Receptor, Notch1 / metabolism

Substances

  • Forkhead Transcription Factors
  • Gsdma3 protein, mouse
  • Homeodomain Proteins
  • Hoxc13 protein, mouse
  • MSX2 protein
  • Notch1 protein, mouse
  • Proteins
  • Receptor, Notch1
  • Whn protein