Identification of a cyclin B1-derived CTL epitope eliciting spontaneous responses in both cancer patients and healthy donors

Cancer Immunol Immunother. 2011 Feb;60(2):227-34. doi: 10.1007/s00262-010-0933-y. Epub 2010 Oct 28.


With the aim to identify cyclin B1-derived peptides with high affinity for HLA-A2, we used three in silico prediction algorithms to screen the protein sequence for possible HLA-A2 binders. One peptide scored highest in all three algorithms, and the high HLA-A2-binding affinity of this peptide was verified in an HLA stabilization assay. By stimulation with peptide-loaded dendritic cells a CTL clone was established, which was able to kill two breast cancer cell lines in an HLA-A2-dependent and peptide-specific manner, demonstrating presentation of the peptide on the surface of cancer cells. Furthermore, blood from cancer patients and healthy donors was screened for spontaneous T-cell reactivity against the peptide in IFN-γ ELISPOT assays. Patients with breast cancer, malignant melanoma, or renal cell carcinoma hosted powerful and high-frequency T-cell responses against the peptide. In addition, when blood from healthy donors was tested, similar responses were observed. Ultimately, serum from cancer patients and healthy donors was analyzed for anti-cyclin B1 antibodies. Humoral responses against cyclin B1 were frequently detected in both cancer patients and healthy donors. In conclusion, a high-affinity cyclin B1-derived HLA-A2-restricted CTL epitope was identified, which was presented on the cell surface of cancer cells, and elicited spontaneous T-cell responses in cancer patients and healthy donors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Algorithms
  • Antibodies / blood*
  • Antibodies / immunology*
  • Cells, Cultured
  • Cyclin B1 / chemistry
  • Cyclin B1 / immunology*
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes, T-Lymphocyte / immunology*
  • HLA-A2 Antigen / immunology
  • Health*
  • Humans
  • Middle Aged
  • Neoplasms / blood
  • Neoplasms / immunology*
  • T-Lymphocytes, Cytotoxic / immunology*


  • Antibodies
  • CCNB1 protein, human
  • Cyclin B1
  • Epitopes, T-Lymphocyte
  • HLA-A2 Antigen