Tobacco genes encoding acidic and basic isoforms of pathogenesis-related proteins display different expression patterns

Plant Mol Biol. 1990 Feb;14(2):119-26. doi: 10.1007/BF00018553.

Abstract

The induction by cytokinin stress and ethylene of nine different tobacco mosaic virus-inducible mRNA classes (termed A-I) encoding pathogenesis-related (PR) proteins was studied. The induced mRNA levels were compared to basal levels in healthy tobacco plants grown in tissue culture and in a greenhouse. Cytokinin stress and ethylene were found to induce different subsets of the mRNAs, indicating that ethylene is not the primary inducing signal in cytokinin-stressed shoots. mRNAs F, H and G encoding the basic hydrolytic enzymes chitinase, beta-1,3-glucanase and a basic equivalent of PR-1, respectively, were found to be expressed at high levels in roots of healthy plants. mRNAs D, I and B encoding the acidic equivalents of the proteins proved to be present at low levels in healthy plants. These results indicate that genes encoding basic and acidic isoforms of pathogenesis-related proteins are differentially regulated.

MeSH terms

  • Cloning, Molecular
  • DNA, Bacterial / genetics
  • Gene Expression
  • Genes, Plant*
  • Light
  • Nucleic Acid Hybridization
  • Plant Proteins / genetics*
  • Plants, Toxic*
  • RNA, Messenger / genetics
  • RNA, Messenger / isolation & purification
  • Tobacco / genetics*
  • Tobacco / microbiology
  • Tobacco Mosaic Virus / genetics*
  • Tobacco Mosaic Virus / pathogenicity
  • Transformation, Genetic

Substances

  • DNA, Bacterial
  • Plant Proteins
  • RNA, Messenger
  • T-DNA
  • pathogenesis-related proteins, plant