Chemical synthesis of oligoribonucleotides for structural studies

Nucleic Acids Symp Ser. 1990:(22):43-4.

Abstract

Large-scale synthesis of oligoribonucleotides has been performed successfully on a solid support by the phosphoramidite approach using levulinyl and tetrahydrofuranyl protection for the 5'- and 2'-hydroxyl groups respectively. A hexamer containing inosine and four fragments of a hammerhead-type ribozyme have been synthesized on a 10 mumol scale for structural studies by NMR spectroscopy and X-ray crystallography.

MeSH terms

  • Base Sequence
  • Molecular Sequence Data
  • Molecular Structure
  • Oligoribonucleotides / chemical synthesis*
  • Oligoribonucleotides / chemistry
  • RNA, Catalytic / chemistry

Substances

  • Oligoribonucleotides
  • RNA, Catalytic