Quantitative assay for bradykinin in rat plasma by liquid chromatography coupled to tandem mass spectrometry

J Pharm Biomed Anal. 2011 Feb 20;54(3):557-61. doi: 10.1016/j.jpba.2010.09.041. Epub 2010 Oct 8.


An assay to quantify bradykinin in rat plasma has been developed and validated, using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Sar-D-Phe(8)-des-Arg(9)-bradykinin was used as internal standard. Aprotinin was added to rat plasma to inhibit the activity of proteinases. Recoveries for solid-phase extraction (SPE) on Strata X reversed phase were greater than 80%. Multiple reaction monitoring (MRM) on a triple quadrupole mass spectrometer equipped with an electrospray source (ESI), operating in the positive ion-mode, was used for detection. The assay was validated and stability was explored. Bradykinin (10-500 ng/mL) was quantified with accuracy values (% RE) below 10% and intra- and inter-day precisions (% RSD) below 12 and 16%, respectively, for all concentrations. The method was successfully applied to several plasma samples from low levels kallikrein rats (LKRs) compared with normal kallikrein rats (NKRs).

Publication types

  • Validation Study

MeSH terms

  • Animals
  • Aprotinin / metabolism
  • Bradykinin / blood*
  • Chromatography, High Pressure Liquid
  • Chromatography, Liquid
  • Kallikreins / urine
  • Mass Spectrometry
  • Rats
  • Rats, Wistar
  • Reproducibility of Results
  • Serine Proteinase Inhibitors / metabolism
  • Solid Phase Extraction
  • Spectrometry, Mass, Electrospray Ionization
  • Tandem Mass Spectrometry
  • Vasodilator Agents / blood*


  • Serine Proteinase Inhibitors
  • Vasodilator Agents
  • Aprotinin
  • Kallikreins
  • Bradykinin