First certified reference material for cystatin C in human serum ERM-DA471/IFCC

Clin Chem Lab Med. 2010 Nov;48(11):1619-21. doi: 10.1515/CCLM.2010.318. Epub 2010 Oct 29.


The IFCC Working Group for the Standardisation of Cystatin C (WG-SCC), in collaboration with the Institute for Reference Materials and Measurements (IRMM), announces the availability of the new certified reference material ERM-DA471/IFCC. The material was characterised using a pure protein primary reference preparation (PRP) as calibrant. The PRP was prepared from recombinant cystatin C, and its concentration measured using dry mass determination. The characterisation of ERM-DA471/IFCC was performed by particle enhanced immuno-nephelometry, particle enhanced immuno-turbidimetry, and enzyme amplified single radial immuno-diffusion. The certified cystatin C mass concentration in ERM-DA471/IFCC, if reconstituted according to the specified procedure, is 5.48 mg/L, the expanded uncertainty (k=2) being 0.15 mg/L.

MeSH terms

  • Blood Chemical Analysis / standards*
  • Calibration
  • Cystatin C / blood*
  • Humans
  • International Agencies*
  • Reference Standards


  • Cystatin C