The beta-tubulin gene family in Zea mays: two differentially expressed beta-tubulin genes

Plant Mol Biol. 1990 Dec;15(6):957-72. doi: 10.1007/BF00039438.

Abstract

Maize beta-tubulins are encoded by a large multigene family with at least nine members, as determined by Southern blot analysis. Two expressed genes, represented by the beta 1 genomic clone and the beta 2 cDNA clone, were examined in this study. The two genes encode beta-tubulins which show 94% sequence identity at the amino acid level. Maize beta 1 transcript levels were highest in seedling root tip and tissue culture cells, which are both rapidly dividing tissues. No transcripts were detected in non-dividing leaf tissue. In contrast, beta 2 transcripts were present at relatively high levels in tissue culture cells and at lower levels in seedling root tip and leaf tissue. The electrophoretic mobility of the beta 2 polypeptide was examined in relation to the constellation of beta-tubulin polypeptides on two-dimensional gel western blots of a maize pollen total protein extract. No evidence for post-translational modification of the beta-tubulin polypeptides was found in pollen.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Base Sequence
  • DNA / genetics
  • Gene Expression Regulation*
  • Genes, Plant*
  • Microtubules / ultrastructure
  • Molecular Sequence Data
  • Multigene Family*
  • Organ Specificity
  • Plant Proteins / biosynthesis
  • Plant Proteins / genetics*
  • Pollen
  • Seeds
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic
  • Tubulin / biosynthesis
  • Tubulin / genetics*
  • Zea mays / genetics*

Substances

  • Plant Proteins
  • Tubulin
  • DNA

Associated data

  • GENBANK/X52878
  • GENBANK/X52879