Designing a highly active soluble PQQ-glucose dehydrogenase for efficient glucose biosensors and biofuel cells

Biochem Biophys Res Commun. 2010 Nov 26;402(4):750-4. doi: 10.1016/j.bbrc.2010.10.102. Epub 2010 Oct 29.


We report for the first time a soluble PQQ-glucose dehydrogenase that is twice more active than the wild type for glucose oxidation and was obtained by combining site directed mutagenesis, modelling and steady-state kinetics. The observed enhancement is attributed to a better interaction between the cofactor and the enzyme leading to a better electron transfer. Electrochemical experiments also demonstrate the superiority of the new mutant for glucose oxidation and make it a promising enzyme for the development of high-performance glucose biosensors and biofuel cells.

MeSH terms

  • Acinetobacter calcoaceticus / enzymology*
  • Bioelectric Energy Sources*
  • Biosensing Techniques*
  • Glucose / analysis*
  • Glucose Dehydrogenases / chemistry*
  • Glucose Dehydrogenases / genetics
  • Mutagenesis
  • Mutation
  • Protein Conformation
  • Protein Engineering


  • Glucose Dehydrogenases
  • glucose dehydrogenase (pyrroloquinoline-quinone)
  • Glucose