Distinct roles of multiple NDH-1 complexes in the cyanobacterial electron transport network as revealed by kinetic analysis of P700+ reduction in various Ndh-deficient mutants of Synechocystis sp. strain PCC6803

J Bacteriol. 2011 Jan;193(1):292-5. doi: 10.1128/JB.00984-10. Epub 2010 Oct 29.

Abstract

While methyl viologen had only a small effect on P700(+) rereduction kinetics after far-red pulses in KCN-treated wild-type Synechocystis sp. strain PCC6803 and an NdhF3/NdhF4 (NdhF3/F4)-defective mutant, it involved a rather slow P700(+) rereduction in an NdhF1-defective mutant. This strongly indicates that (i) active electron flow from metabolites to plastoquinone is suppressed upon deletion of ndhF1 and (ii) photosystem 1-mediated cyclic electron transport is dependent on NdhF3/F4-type NDH-1 complexes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Cell Respiration / physiology
  • Electron Transport / physiology*
  • Gene Expression Regulation, Bacterial / physiology*
  • Kinetics
  • Mutation
  • NADPH Dehydrogenase / genetics
  • NADPH Dehydrogenase / metabolism*
  • Oxidation-Reduction
  • Paraquat / pharmacology
  • Synechocystis / genetics
  • Synechocystis / metabolism*

Substances

  • Bacterial Proteins
  • NADPH Dehydrogenase
  • Paraquat