Rapid Blue-Light-Mediated Induction of Protein Interactions in Living Cells

Nat Methods. 2010 Dec;7(12):973-5. doi: 10.1038/nmeth.1524. Epub 2010 Oct 31.

Abstract

Dimerizers allowing inducible control of protein-protein interactions are powerful tools for manipulating biological processes. Here we describe genetically encoded light-inducible protein-interaction modules based on Arabidopsis thaliana cryptochrome 2 and CIB1 that require no exogenous ligands and dimerize on blue-light exposure with subsecond time resolution and subcellular spatial resolution. We demonstrate the utility of this system by inducing protein translocation, transcription and Cre recombinase-mediated DNA recombination using light.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arabidopsis / genetics*
  • Arabidopsis / metabolism
  • Arabidopsis / radiation effects
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Arabidopsis Proteins / radiation effects
  • Basic Helix-Loop-Helix Transcription Factors / metabolism
  • Basic Helix-Loop-Helix Transcription Factors / radiation effects
  • Cryptochromes / metabolism
  • Cryptochromes / radiation effects
  • Kinetics
  • Light*
  • Mammals
  • Recombinant Fusion Proteins / metabolism
  • Recombinant Fusion Proteins / radiation effects
  • Saccharomyces cerevisiae / genetics
  • Transcription, Genetic / radiation effects

Substances

  • Arabidopsis Proteins
  • Basic Helix-Loop-Helix Transcription Factors
  • CIB1 protein, Arabidopsis
  • CRY2 protein, Arabidopsis
  • Cryptochromes
  • Recombinant Fusion Proteins