Antitumor effects of nano-bubble hydrogen-dissolved water are enhanced by coexistent platinum colloid and the combined hyperthermia with apoptosis-like cell death

Oncol Rep. 2010 Dec;24(6):1463-70. doi: 10.3892/or_00001006.


In order to erase reactive oxygen species (ROS) related with the proliferation of tumor cells by reducing activity of hydrogen, we developed functional water containing nano-bubbles (diameters: <900 nm for 71%/population) hydrogen of 1.1-1.5 ppm (the theoretical maximum: 1.6 ppm) with a reducing ability (an oxidation-reduction potential -650 mV, normal water: +100-200 mV) using a microporous-filter hydrogen-jetting device. We showed that hydrogen water erased ROS indispensable for tumor cell growth by ESR/spin trap, the redox indicator CDCFH-DA assay, and was cytotoxic to Ehrlich ascites tumor cells as assessed by WST-8 assay, crystal violet dye stain and scanning electron microscopy, after 24-h or 48-h incubation sequent to warming at 37°C or 42°C. Hydrogen water supplemented with platinum colloid (0.3 ppm Pt in 4% polyvinylpyrrolidone) had more antitumor activity than hydrogen water alone, mineral water alone (15.6%), hydrogen water plus mineral water, or platinum colloid alone as observed by decreased cell numbers, cell shrinkage and pycnosis (nuclear condensation)/karyorrhexis (nuclear fragmentation) indicative of apoptosis, together with cell deformation and disappearance of microvilli on the membrane surface. These antitumor effects were promoted by combination with hyperthermia at 42°C. Thus, the nano-bubble hydrogen water with platinum colloid is potent as an anti-tumor agent.

Publication types

  • Evaluation Study

MeSH terms

  • Antineoplastic Combined Chemotherapy Protocols / administration & dosage
  • Antineoplastic Combined Chemotherapy Protocols / chemistry
  • Antineoplastic Combined Chemotherapy Protocols / pharmacology
  • Apoptosis* / drug effects
  • Cell Death / drug effects
  • Cells, Cultured
  • Colloids / administration & dosage
  • Colloids / pharmacology
  • Combined Modality Therapy
  • Drug Synergism
  • Gases / administration & dosage
  • Gases / chemistry
  • Gases / pharmacology
  • Humans
  • Hydrogen / administration & dosage*
  • Hydrogen / chemistry
  • Hydrogen / pharmacology
  • Hyperthermia, Induced* / methods
  • Models, Biological
  • Nanoparticles
  • Neoplasms / pathology
  • Neoplasms / therapy*
  • Platinum / administration & dosage*
  • Platinum / pharmacology
  • Solubility
  • Water / administration & dosage*
  • Water / chemistry
  • Water / pharmacology


  • Colloids
  • Gases
  • Water
  • Platinum
  • Hydrogen