Impedance-based flow cytometry for the measurement of microparticles

Semin Thromb Hemost. 2010 Nov;36(8):819-23. doi: 10.1055/s-0030-1267035. Epub 2010 Nov 3.


Over the last several years, there has been considerable interest in evaluating the biological relevance of alterations in blood-borne microparticle populations. The most commonly employed technique for the characterization of microparticles is light scatter flow cytometry. However, the enumeration and sizing of submicron particles based on light scattering properties can be problematic. Impedance-based flow cytometry based on the Coulter principle offers a sensitive methodology to characterize microparticles. This review details the rationale for employing impedance-based flow cytometry in the measurement of blood-borne microparticles.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Animals
  • Blood Cells / ultrastructure
  • Cell-Derived Microparticles*
  • Electric Impedance
  • Flow Cytometry / instrumentation
  • Flow Cytometry / methods*
  • Humans
  • Particle Size
  • Scattering, Radiation