Akt2 Regulates Expression of the Actin-Bundling Protein Palladin

FEBS Lett. 2010 Dec 1;584(23):4769-74. doi: 10.1016/j.febslet.2010.10.056. Epub 2010 Nov 2.

Abstract

The phosphatidylinositol 3-kinase/Akt pathway is responsible for key aspects of tumor progression, and is frequently hyperactivated in cancer. We have recently identified palladin, an actin-bundling protein that functions to control the actin cytoskeleton, as an Akt1-specific substrate that inhibits breast cancer cell migration. Here we have identified a role for Akt isoforms in the regulation of palladin expression. Akt2, but not Akt1, enhances palladin expression by maintaining protein stability and upregulating transcription. These data reveal that Akt signaling regulates the stability of palladin, and further supports the notion that Akt isoforms have distinct and specific roles in tumorigenesis.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / chemistry*
  • Actins / metabolism*
  • Cell Line
  • Cytoskeletal Proteins / chemistry
  • Cytoskeletal Proteins / genetics*
  • Cytoskeletal Proteins / metabolism*
  • Gene Expression Regulation*
  • Humans
  • Isoenzymes / metabolism
  • Phosphoproteins / chemistry
  • Phosphoproteins / genetics*
  • Phosphoproteins / metabolism*
  • Protein Multimerization*
  • Protein Stability
  • Protein Structure, Quaternary
  • Proto-Oncogene Proteins c-akt / metabolism*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Up-Regulation

Substances

  • Actins
  • Cytoskeletal Proteins
  • Isoenzymes
  • Phosphoproteins
  • RNA, Messenger
  • palladin protein, human
  • Proto-Oncogene Proteins c-akt