Modulation of PARP-1 and PARP-2 expression by L-carnosine and trehalose after LPS and INFγ-induced oxidative stress

Neurochem Res. 2010 Dec;35(12):2144-53. doi: 10.1007/s11064-010-0297-x. Epub 2010 Oct 30.


Poly(ADP-ribose) polymerases (PARPs) play a crucial role in DNA damage surveillance through their nick sensor functions. Since PARPs' over activation leads to an excessive consumption of NAD(+) and ATP depletion, these enzymes also are involved in the early events of programmed cell death as well as in necrosis. In order to verify the protective action of L: -carnosine and trehalose against NO induced cell death, in the present study we examined their effects on the expression of PARP-1, PARP-2 and iNOS in primary rat astrocyte and oligodendrocyte cells, treated with lipopolysaccharide (LPS) and interferon gamma (INFγ), through semi-quantitative PCR and western analysis. To further characterize the molecular mechanisms underlying L-carnosine and trehalose action, we measured cell viability, nitrite production and LDH release. The data obtained clearly demonstrate that in the stress model employed L-carnosine and trehalose down regulate PARP-1 and PARP-2 expression in both cell phenotypes, thus suggesting their possible application in clinical trials.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Astrocytes / drug effects
  • Astrocytes / metabolism
  • Base Sequence
  • Blotting, Western
  • Carnosine / pharmacology*
  • Cells, Cultured
  • DNA Primers
  • Interferon-gamma / pharmacology*
  • Lipopolysaccharides / pharmacology*
  • Oligodendroglia / drug effects
  • Oligodendroglia / metabolism
  • Oxidative Stress
  • Poly (ADP-Ribose) Polymerase-1
  • Poly(ADP-ribose) Polymerases / metabolism*
  • Polymerase Chain Reaction
  • Rats
  • Trehalose / pharmacology*


  • DNA Primers
  • Lipopolysaccharides
  • Interferon-gamma
  • Carnosine
  • Trehalose
  • Parp1 protein, rat
  • Parp2 protein, rat
  • Poly (ADP-Ribose) Polymerase-1
  • Poly(ADP-ribose) Polymerases