The F-BAR domain of SRGP-1 facilitates cell-cell adhesion during C. elegans morphogenesis

J Cell Biol. 2010 Nov 15;191(4):761-9. doi: 10.1083/jcb.201005082. Epub 2010 Nov 8.

Abstract

Robust cell-cell adhesion is critical for tissue integrity and morphogenesis, yet little is known about the molecular mechanisms controlling cell-cell junction architecture and strength. We discovered that SRGP-1 is a novel component of cell-cell junctions in Caenorhabditis elegans, localizing via its F-BAR (Bin1, Amphiphysin, and RVS167) domain and a flanking 200-amino acid sequence. SRGP-1 activity promotes an increase in membrane dynamics at nascent cell-cell contacts and the rapid formation of new junctions; in addition, srgp-1 loss of function is lethal in embryos with compromised cadherin-catenin complexes. Conversely, excess SRGP-1 activity leads to outward bending and projections of junctions. The C-terminal half of SRGP-1 interacts with the N-terminal F-BAR domain and negatively regulates its activity. Significantly, in vivo structure-function analysis establishes a role for the F-BAR domain in promoting rapid and robust cell adhesion during embryonic closure events, independent of the Rho guanosine triphosphatase-activating protein domain. These studies establish a new role for this conserved protein family in modulating cell-cell adhesion.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Caenorhabditis elegans Proteins / genetics
  • Caenorhabditis elegans Proteins / metabolism*
  • Caenorhabditis elegans* / cytology
  • Caenorhabditis elegans* / embryology
  • Caenorhabditis elegans* / physiology
  • Cell Adhesion / physiology*
  • Humans
  • Intercellular Junctions / chemistry
  • Intercellular Junctions / metabolism*
  • Morphogenesis / physiology*
  • Protein Structure, Tertiary
  • RNA Interference
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Transgenes
  • alpha Catenin / genetics
  • alpha Catenin / metabolism
  • beta Catenin / genetics
  • beta Catenin / metabolism

Substances

  • Caenorhabditis elegans Proteins
  • Recombinant Fusion Proteins
  • alpha Catenin
  • beta Catenin