T cell activation in the elderly: evidence for specific deficiencies in T cell/accessory cell interactions

Mech Ageing Dev. 1990 Feb 15;51(3):265-76. doi: 10.1016/0047-6374(90)90076-r.


We studied T cell activation in the healthy aged (greater than 70 years) by examining lymphocyte proliferative responses to various mitogenic stimuli in accessory cell (AC)-dependent and AC-independent systems. Results show that despite a near normal response to the anti-CD3 monoclonal antibody (mAb) OKT3, peripheral blood mononuclear cells (PBM) from the elderly exhibit a profound reduction in phytohaemagglutinin (PHA)-responsiveness (approximately 30% of young adults). This deficit becomes even more severe at suboptimal doses of PHA. Adding exogenous interleukin-2 (IL-2) or pretreating the AC population with gamma interferon (IFN-gamma) returns the level of proliferation to that seen with young adults. Furthermore, replacing "old" AC with AC from young adults or with U937 (a monocytic cell line) in T cell/AC cell-mixing experiments restores PHA-responsiveness in 70% of cases. On the other hand, AC from the aged fully support PHA responses in T cells from young adults. In AC-depleted cultures, purified T cells from the aged respond normally to the co-mitogenic stimuli, PHA + PMA. Taken together, these results suggest that the age-associated diminution in PHA-responsiveness is due, at least in part, to specific deficiencies in T cell/AC communication.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Aging / immunology*
  • Antibodies, Monoclonal / immunology
  • Antigen-Presenting Cells / physiology*
  • Cell Communication
  • Cell Division / physiology
  • Female
  • Humans
  • In Vitro Techniques
  • Interferon-gamma / immunology
  • Interleukin-2 / immunology
  • Lymphocyte Activation
  • Male
  • Phytohemagglutinins / immunology
  • T-Lymphocytes / physiology*


  • Antibodies, Monoclonal
  • Interleukin-2
  • Phytohemagglutinins
  • Interferon-gamma