Hydrogen-rich saline provides protection against hyperoxic lung injury

J Surg Res. 2011 Jan;165(1):e43-9. doi: 10.1016/j.jss.2010.09.024. Epub 2010 Oct 15.


Background: Hydrogen has been proven to be a novel antioxidant through its selectively reducing of the hydroxyl radical. In this study, we investigated the effects of hydrogen-rich saline on the prevention of acute lung injury induced by hyperoxia (HALI) in rats.

Materials and methods: Physiologic saline, hydrogen-rich saline, or nitrogen-rich saline was administered through intraperitoneal (i.p.) injection during exposure to hyperoxia (10 mL/Kg), respectively.

Results: Severity of HALI was assessed by the volume of pleural effusion, wet-to-dry weight ratio (W/D), and histologic analysis. Apoptosis in lung cells was determined with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-positive staining. The content of pro-inflammatory cytokine interleukin IL-1b and TNF-a in the lung tissues were detected by enzyme-linked immunosorbent assay (ELISA). Hydrogen-rich saline treatment provides protection against HALI by inhibiting lipid, DNA oxidation, and tissue edema. Moreover, hydrogen-rich saline treatment could inhibit apoptosis and inflammation while no significant reduction was observed in nitrogen-rich saline treated animals.

Conclusion: The results of this study demonstrate that hydrogen-rich saline ameliorated hyperoxia-induced acute lung injury by reducing oxidative stress and inflammatory cascades in lung tissue.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Animals
  • Apoptosis
  • Deoxyguanosine / analogs & derivatives
  • Deoxyguanosine / analysis
  • Hydrogen / therapeutic use*
  • Hyperoxia / complications*
  • Lung / pathology
  • Lung Injury / prevention & control*
  • Male
  • Malondialdehyde / analysis
  • Peroxidase / analysis
  • Pulmonary Edema / prevention & control
  • Rats
  • Rats, Sprague-Dawley
  • Sodium Chloride / therapeutic use*
  • Superoxide Dismutase / analysis


  • Sodium Chloride
  • Malondialdehyde
  • Hydrogen
  • 8-Hydroxy-2'-Deoxyguanosine
  • Peroxidase
  • Superoxide Dismutase
  • Deoxyguanosine