Negative association of Epstein-Barr virus or herpes simplex virus-1 with tumefactive central nervous system inflammatory demyelinating disease

J Neurovirol. 2010 Nov;16(6):466-71. doi: 10.1007/BF03210852. Epub 2010 Nov 11.


Central nervous system (CNS) demyelination has been suggested to be associated with infections caused by the Epstein-Barr virus (EBV) or herpes simplex virus (HSV)-1. CNS inflammatory demyelinating disease (IDD) rarely presents as a large lesion. We evaluated samples of serum and cerebrospinal fluid (CSF) by enzyme-linked immunosorbent assay to detect recent infection with these viruses and analyzed CSF and brain specimens by polymerase chain reaction (PCR) or immunohistochemical studies for evidence of these viruses in three patients with biopsy-proven CNS IDD. The results of PCR tests for EBV and HSV in CSF or brain specimens were negative. Elevated anti-EBV or -HSV antibody levels were not found in serum or CSF in any patient. Immunohistochemical studies showed that IDD lesions were negative for latent membrane protein (LMP)-1, Epstein-Barr nuclear antigen (EBNA)-2, and EBNA noncoding RNA (EBER)-1. These results suggest a negative association between CNS IDD and EBV or HSV.

MeSH terms

  • Adult
  • Antibodies, Viral / cerebrospinal fluid
  • Central Nervous System / pathology
  • Central Nervous System / virology*
  • Central Nervous System Viral Diseases / cerebrospinal fluid
  • Central Nervous System Viral Diseases / complications*
  • Central Nervous System Viral Diseases / metabolism
  • DNA, Viral / cerebrospinal fluid
  • Epstein-Barr Virus Infections / complications*
  • Female
  • Guillain-Barre Syndrome / cerebrospinal fluid
  • Guillain-Barre Syndrome / complications*
  • Guillain-Barre Syndrome / virology
  • Herpes Simplex / cerebrospinal fluid
  • Herpes Simplex / complications*
  • Herpesvirus 1, Human / pathogenicity
  • Herpesvirus 4, Human / pathogenicity
  • Humans
  • Male
  • Middle Aged
  • Multiple Sclerosis / cerebrospinal fluid
  • Multiple Sclerosis / virology
  • Polymerase Chain Reaction


  • Antibodies, Viral
  • DNA, Viral