AMP-activated protein kinase regulates the expression of monocarboxylate transporter 4 in skeletal muscle

Life Sci. 2011 Jan 17;88(3-4):163-8. doi: 10.1016/j.lfs.2010.11.003. Epub 2010 Nov 8.


Aims: The aim of this study was to determine the effect of 5-aminoimidazole-4-carboxamide-1-β-D-ribofuranoside (AICAR), an AMP-activated protein kinase (AMPK) activator, on monocarboxylate transporter 4 (MCT4) expression in rat skeletal muscle and a prototypic embryonal rhabdomyosarcoma cell line (RD cells).

Main methods: We examined the alteration in Glucose transporter 4 (GLUT4) and MCT4 mRNA levels by quantitative real-time PCR. Alteration in GLUT4 and MCT4 protein levels was examined by Western blotting.

Key findings: In an in vivo study, AICAR increased MCT4 mRNA and protein levels in a fiber-type specific manner. In an in vitro study, AICAR increased MCT4 mRNA and protein levels. Moreover, AICAR-induced MCT4 expression was blocked by Compound C, an AMPK inhibitor.

Significance: In this study, we found that AMPK activation induced expression of MCT4 in RD cells and rat skeletal muscle in a fiber-type specific manner. These results indicate the possible involvement of an AMPK-mediated pathway associated with MCT4 expression in skeletal muscle.

MeSH terms

  • AMP-Activated Protein Kinases / metabolism*
  • Aminoimidazole Carboxamide / analogs & derivatives*
  • Aminoimidazole Carboxamide / metabolism
  • Aminoimidazole Carboxamide / pharmacology
  • Analysis of Variance
  • Animals
  • Blotting, Western
  • Cell Line, Tumor
  • DNA Primers / genetics
  • Gene Expression Regulation / genetics
  • Gene Expression Regulation / physiology*
  • Glucose / pharmacokinetics
  • Glucose Transporter Type 4 / metabolism
  • Male
  • Monocarboxylic Acid Transporters / metabolism*
  • Muscle Proteins / metabolism*
  • Muscle, Skeletal / metabolism*
  • Rats
  • Rats, Wistar
  • Reverse Transcriptase Polymerase Chain Reaction
  • Ribonucleotides / metabolism*
  • Ribonucleotides / pharmacology


  • DNA Primers
  • Glucose Transporter Type 4
  • Monocarboxylic Acid Transporters
  • Muscle Proteins
  • Ribonucleotides
  • Slc16a3 protein, rat
  • Aminoimidazole Carboxamide
  • AMP-Activated Protein Kinases
  • AICA ribonucleotide
  • Glucose