Use of a Gluconobacter frateurii mutant to prevent dihydroxyacetone accumulation during glyceric acid production from glycerol

Biosci Biotechnol Biochem. 2010;74(11):2330-2. doi: 10.1271/bbb.100406. Epub 2010 Nov 7.


To prevent dihydroxyacetone (DHA) by-production during glyceric acid (GA) production from glycerol using Gluconobacter frateurii, we used a G. frateurii THD32 mutant, ΔsldA, in which the glycerol dehydrogenase subunit-encoding gene (sldA) was disrupted, but ΔsldA grew much more slowly than the wild type, growth starting after a lag of 3 d under the same culture conditions. The addition of 1% w/v D-sorbitol to the medium improved both the growth and the GA productivity of the mutant, and ΔsldA produced 89.1 g/l GA during 4 d of incubation without DHA accumulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Dihydroxyacetone / metabolism*
  • Gluconobacter / genetics
  • Gluconobacter / growth & development
  • Gluconobacter / metabolism*
  • Glyceric Acids / metabolism*
  • Glycerol / metabolism*
  • Mutant Proteins / metabolism*
  • Mutation
  • Sugar Alcohol Dehydrogenases / genetics


  • Glyceric Acids
  • Mutant Proteins
  • glyceric acid
  • Sugar Alcohol Dehydrogenases
  • glycerol dehydrogenase
  • Dihydroxyacetone
  • Glycerol