Chitosan-induced stomatal closure accompanied by peroxidase-mediated reactive oxygen species production in Arabidopsis

Biosci Biotechnol Biochem. 2010;74(11):2313-5. doi: 10.1271/bbb.100340. Epub 2010 Nov 7.


Chitosan induced stomatal closure in wild type-plants and NADPH oxidase knock-out mutants (atrbohD atrbohF), and reactive oxygen species (ROS) production in wild-type guard cells. Closure and production were completely abolished by catalase and a peroxidase inhibitor. These results indicate that chitosan induces ROS production mediated by peroxidase, resulting in stomatal closure.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / metabolism*
  • Catalase
  • Chitosan / pharmacology*
  • Gene Knockout Techniques
  • NADPH Oxidases / genetics
  • Peroxidase / metabolism*
  • Plant Stomata / drug effects*
  • Plant Stomata / metabolism
  • Reactive Oxygen Species / pharmacology*


  • Reactive Oxygen Species
  • Chitosan
  • Catalase
  • Peroxidase
  • NADPH Oxidases