Expression patterns of ABCG2, Bmi-1, Oct-3/4, and Yap in the developing mouse incisor

Gene Expr Patterns. Mar-Apr 2011;11(3-4):163-70. doi: 10.1016/j.gep.2010.11.001. Epub 2010 Nov 10.

Abstract

Recent studies have demonstrated the existence of dental stem cells in the continuously growing tooth. However, much remains to be learned about the complex mechanism involving stem cells during tooth development. We determined the expression patterns of four stem cell markers ABCG2, Bmi-1, Oct-3/4, and Yap in the developing mouse incisors between embryonic day (E) 11 and postnatal day (PN) 20. ABCG2 was localized strongly in the perivascular region of the incisor mesenchyme from E11 to PN20, and in the odontoblasts from E18 to PN20. Bmi-1 was expressed in both the dental epithelium and mesenchyme from E11 to E14. The expression of Bmi-1 was noticeably reduced at E16, and was restricted to the apical bud from E16 to PN20. Oct-3/4 was localized in the nucleus of the cells in the superficial layer and stellate reticulum within the dental epithelium from E11 to E14 and in the apical bud from E16 to PN20. Meanwhile, once the ameloblasts and odontoblasts began to appear at E16, they expressed Oct-3/4 in the cytoplasm. Yap was expressed in most of the basal cells of the incisor dental epithelium from E11 to E14, but was expressed mainly in the transit-amplifying (TA) cells within the basal cell layer from E16 to PN20. The unique and overlapping expression patterns of ABCG2, Bmi-1, Oct-3/4, and Yap suggest the independent and interactive functions of the four stem cell markers in the developing mouse incisor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily G, Member 2
  • ATP-Binding Cassette Transporters / genetics*
  • ATP-Binding Cassette Transporters / metabolism
  • Adaptor Proteins, Signal Transducing / genetics*
  • Adaptor Proteins, Signal Transducing / metabolism
  • Animals
  • Antigens, Differentiation / metabolism
  • Cell Cycle Proteins
  • Dental Papilla / embryology
  • Dental Papilla / growth & development
  • Dental Papilla / metabolism
  • Embryonic Stem Cells / metabolism
  • Epithelium / embryology
  • Epithelium / growth & development
  • Epithelium / metabolism
  • Female
  • Gene Expression Regulation, Developmental
  • Incisor / embryology*
  • Incisor / growth & development*
  • Incisor / metabolism
  • Mice
  • Mice, Inbred ICR
  • Nuclear Proteins / genetics*
  • Nuclear Proteins / metabolism
  • Octamer Transcription Factor-3 / genetics*
  • Octamer Transcription Factor-3 / metabolism
  • Phosphoproteins / genetics*
  • Phosphoproteins / metabolism
  • Polycomb Repressive Complex 1
  • Proto-Oncogene Proteins / genetics*
  • Proto-Oncogene Proteins / metabolism
  • Repressor Proteins / genetics*
  • Repressor Proteins / metabolism

Substances

  • ATP Binding Cassette Transporter, Subfamily G, Member 2
  • ATP-Binding Cassette Transporters
  • Abcg2 protein, mouse
  • Adaptor Proteins, Signal Transducing
  • Antigens, Differentiation
  • Bmi1 protein, mouse
  • Cell Cycle Proteins
  • Nuclear Proteins
  • Octamer Transcription Factor-3
  • Phosphoproteins
  • Proto-Oncogene Proteins
  • Repressor Proteins
  • Yap protein, mouse
  • Polycomb Repressive Complex 1