A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene

Cell. 2010 Nov 12;143(4):628-38. doi: 10.1016/j.cell.2010.09.047.


Worldwide, acute, and chronic pain affects 20% of the adult population and represents an enormous financial and emotional burden. Using genome-wide neuronal-specific RNAi knockdown in Drosophila, we report a global screen for an innate behavior and identify hundreds of genes implicated in heat nociception, including the α2δ family calcium channel subunit straightjacket (stj). Mice mutant for the stj ortholog CACNA2D3 (α2δ3) also exhibit impaired behavioral heat pain sensitivity. In addition, in humans, α2δ3 SNP variants associate with reduced sensitivity to acute noxious heat and chronic back pain. Functional imaging in α2δ3 mutant mice revealed impaired transmission of thermal pain-evoked signals from the thalamus to higher-order pain centers. Intriguingly, in α2δ3 mutant mice, thermal pain and tactile stimulation triggered strong cross-activation, or synesthesia, of brain regions involved in vision, olfaction, and hearing.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Animals
  • Back Pain / genetics
  • Calcium Channels / genetics*
  • Calcium Channels / metabolism
  • Drosophila / genetics*
  • Drosophila Proteins / genetics*
  • Drosophila Proteins / metabolism
  • Gene Knockdown Techniques
  • Genome-Wide Association Study
  • Hot Temperature
  • Humans
  • Mice
  • Pain / genetics*
  • Polymorphism, Single Nucleotide
  • RNA Interference


  • CACNA2D3 protein, human
  • CACNA2D3 protein, mouse
  • Calcium Channels
  • Drosophila Proteins
  • stj protein, Drosophila