C-factor: A Cell-Cell Signaling Protein Required for Fruiting Body Morphogenesis of M. Xanthus

Cell. 1990 Apr 6;61(1):19-26. doi: 10.1016/0092-8674(90)90211-v.

Abstract

During fruiting body development, the product of the csgA gene is necessary for cellular aggregation, for spore differentiation, and for gene expression that is initiated after 6 hr of starvation. From nascent wild-type fruiting bodies we have purified a polypeptide of 17 kd called C-factor, which, at approximately 1 to 2 nM, restores normal development to csgA mutant cells. C-factor activity is not recovered from extracts of unstarved, growing cells or csgA mutant cells. The amino acid sequence from purified C-factor demonstrates that it is the product of the csgA gene. C-factor is active over a narrow range of concentration and has properties of a morphogenetic paracrine signal.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / biosynthesis
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / isolation & purification
  • Gene Expression Regulation, Bacterial*
  • Genotype
  • Kinetics
  • Molecular Sequence Data
  • Molecular Weight
  • Morphogenesis
  • Mutation
  • Myxococcales / genetics*
  • Myxococcales / growth & development
  • Signal Transduction

Substances

  • Bacterial Proteins
  • CsgA protein, Myxococcus xanthus