Abstract
The S-peptide and S-protein components of bovine pancreatic ribonuclease form a noncovalent complex with restored ribonucleolytic activity. Although this archetypal protein-fragment complementation system has been the object of historic work in protein chemistry, intrinsic limitations compromise its utility. Modern methods are shown to overcome those limitations and enable new applications.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Bacillus subtilis / enzymology*
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Cattle
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Dexfenfluramine
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Models, Molecular
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Peptide Fragments / chemistry
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Peptide Fragments / metabolism
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Protein Conformation
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Ribonuclease, Pancreatic / chemistry*
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Ribonuclease, Pancreatic / metabolism*
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Subtilisin / metabolism*
Substances
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Peptide Fragments
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Dexfenfluramine
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Ribonuclease, Pancreatic
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Subtilisin