Viability of Human Periodontal Ligament Fibroblasts in Milk, Hank's Balanced Salt Solution and Coconut Water as Storage Media

Int Endod J. 2011 Feb;44(2):111-5. doi: 10.1111/j.1365-2591.2010.01809.x. Epub 2010 Nov 17.


Aim: To evaluate the effectiveness of various storage media at 5 °C for maintaining the viability of human periodontal ligament fibroblasts (PDLF).

Methodology: Plates with PDLF were soaked in recently prepared Hank's balanced salt solution (HBSS), skimmed milk, whole milk, Save-A-Tooth(®) system's HBSS (Save), natural coconut water, industrialized coconut water or tap water (negative control) at 5 °C for 3, 6, 24, 48, 72, 96 and 120 h. Minimum essential medium (MEM) at 37 °C served as the positive control. PDL cell viability was determined by MTT assay. Data were statistically analysed by Kruskal-Wallis test complemented by the Scheffé test (α=5%).

Results: The greatest number of viable cells was observed for MEM. Skimmed and whole milk, followed by natural coconut water and HBSS, were the most effective media in maintaining cell viability (P<0.05). From 24 to 120 h, Save, industrialized coconut water and tap water were the worst storage media.

Conclusions: Skimmed and whole milk had the greatest capacity to maintain PDLF viability when compared with natural coconut water, HBSS, Save, industrialized coconut water and tap water.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Animals
  • Cell Survival / drug effects
  • Cells, Cultured
  • Cocos / chemistry
  • Cold Temperature
  • Culture Media / chemistry
  • Culture Media / pharmacology
  • Fibroblasts / cytology
  • Fibroblasts / drug effects*
  • Humans
  • Isotonic Solutions / chemistry
  • Isotonic Solutions / pharmacology
  • Milk / chemistry
  • Organ Preservation Solutions / chemistry
  • Organ Preservation Solutions / pharmacology*
  • Periodontal Ligament / cytology
  • Periodontal Ligament / drug effects*
  • Statistics, Nonparametric
  • Tissue Preservation / methods*
  • Tooth Avulsion / therapy*
  • Tooth*


  • Culture Media
  • Hanks Balanced Salt Solution
  • Isotonic Solutions
  • Organ Preservation Solutions